No significant differences were found between age groups (p = 0.18), gender (p = 0.12), or limb side (p = 0.85). Mean lymphatic occlusion pressure in the upper limb was 86 mm Hg (CI ☓.7 mm Hg, α = 0.5%). We measured the lymphatic occlusion pressure on 30 healthy volunteers (11 men and 19 women). Lymphatic occlusion pressure was determined by observing when lymph flow stopped under the cuff. A NIRFLI guided manual lymphatic drainage technique named "Fill & Flush Drainage Method" was performed during the measurement to promote lymph flow. After obtaining a baseline of the lymph flow without pressure inside the cuff, the cuff was inflated by increments of 10 mm Hg starting at 30 mm Hg. We observed and recorded lymph flow in the upper limb of healthy volunteers through a transparent cuff using near-infrared fluorescence lymphatic imaging. The goal of this study was to see if this method could be used to measure the lymphatic occlusion pressure. The novel method of near-infrared fluorescence lymphatic imaging (NIRFLI) can be used to visualize lymphatic flow in real time. Given its importance in determining the transport capacity of lymphatic vessels, it is crucial to know its value. There are very little scientific data on occlusion pressure for superficial lymphatic collectors.
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